Abstract Gastric cancer is one the most common human malignancies, with an increased incidence year by year. The underlying mechanisms of Claudin 18 (CLDN18) was involvement in patients with gastric cancer remain poorly understood. We therefore investigated the function of CLDN18 in patients with gastric cancer. Blood samples were collected from gastric cancer patients. CLDN18 and CLDN18 expression were measured using Microarray and qPCR. MTT assay, Transwell assay cells, LDH activity and Caspase-3/9 activity and Flow cytometry were used to measure the effects of ARHGAP26 and CLDN18 on cell growth in gastric cancer. We firstly found that CLDN18 expression were increased in patients with gastric cancer. Then, up-regulation of CLDN18 promoted cell growth in gastric cancer. Down-regulation of CLDN18 induced apoptosis in gastric cancer. Cancer-promoting genetic of CLDN18 is compromised by CLDN18-ARHGAP26 in gastric cancer cell. Down-regulation of ARHGAP26 rescues the effects of CLDN18-mediated tumor promotion effects on gastric cancer cell. CLDN18-ARHGAP26 mediated tumor suppressive effects on gastric cancer cells by ABCG2 and ABCB1 pathway. These results provide evidence that serum CLDN18-ARHGAP26 as a biomarker for tumor promoting genetic in gastric cancer via ABCG2 and ABCB1 pathway.

Abstract To analyze the clinical value of turbidity-elimination gout soup combined with external application of traditional Chinese medicine to improve pain and the volume of tophi in patients with gout. A total of 94 patients with gout were selected as the study samples. They were randomly divided into control group treated with colchicine tablets and observation group treated with turbidity-elimination gout soup combined with external application of traditional Chinese medicine. Two groups of patients were compared before and after treatment in terms of pain, volume of tophi, score of TCM symptoms and laboratory indicators. There was no significant difference in NRS score, volume of tophi, redness and swelling of joints, heat and pain and other laboratory indicators between the two groups before treatment (P>0.05); NRS score, tophi, the integral of symptoms and other symptoms, and erythrocyte sedimentation rate, white blood cell count, blood uric acid level and etc. laboratory indexes of patients in the observation group were lower than those of the control group after treatment (P<0.05). Combination will effectively reduce the pain of patients with gout, reduce the volume of tophi, correct clinical symptoms and improve the level of laboratory indicators, with clinical reference and implementation efficiency.

Abstract In this study, we aimed to compare clinical efficacy of laparoscopic surgery and open surgery for colorectal cancer in the Huaihe River Basin in China. A total of 92 patients with colorectal cancer were selected as subjects. Patients were divided into control group (open surgery, n = 27 cases) and test group (laparoscopic surgery, n = 28 cases). Our results showed that the duration of operation (122.54 ± 14.85) min and length of incision (4.51 ± 1.065) cm were shorter in the experimental group than those in the control group. The intraoperative blood loss of the experimental group was (161.12 ± 10.694) ml, which was less than that of the control group (218.53 ± 15.369) ml (P < 0.05). Patients in the experimental group have higher Five-year survival rate and lower incidence of postoperative complications than those in the control group (all P < 0.05). In conclusion, in the treatment of colorectal cancer, laparoscopic surgery has a better clinical effect than open surgery, which is worthy of clinical application.

Abstract Oxygen glucose deprivation/re-oxygenation (OGD/R)-mediated ischemia of kidney is frequently leads to enhanced apoptosis and amplified inflammatory response. Sappanchalcone (Sap) is a flavonoid compound that extracted from Caesalpinia sappan L. with a range of cell-protective activities. Herein, we primarily reconnoitered the influence of Sap in HK-2 cells under OGD/R treatment. In this research, the consequence revealed that Sap might linked with ischemia of kidney. Besides, Sap lessened OGD/R-mediated HK-2 cell injury by boosting cell viability, inhibiting apoptosis and inflammation. In addition, Sap hindered activation of the TNFRSF1A/NF-kB signaling. Moreover, upregulation of TNFRSF1A diminished the repressive influence of Sap on OGD/R-mediated apoptosis and inflammation. In conclusion, Sap declined the OGD/R-induced HK-2 cell injury by downregulation of TNFRSF1A/NF-kB signaling, thereby offered a theoretical basis for the handling of ischemia of kidney.

Abstract The modification of zein with sodium trimetaphosphate (STMP) as a cross-linking agent was studied. The effects of the mass ratio of STMP to zein (RS/Z), pH, reaction temperature and time on the rheological properties of modified zein solution (Z-SP) were evaluated. By using response surface methodology, the optimal modification conditions were also determined as following: RS/Z 0.03, pH 6.8, reaction time 2 h, reaction temperature 25 °C. Under these conditions, the observed shear stress of Z-SP solution was 35.0025 Pa, close to the predicted value. Compared with natural zein, the viscosity of Z-SP increased with the increasing shear rate, which was attributed to the structural changes, as evidenced by circular dichroism (CD) and Fourier transform infrared (FT-IR) analysis.

The objective of this study was to construct three-dimensional (3D) images of premolars to measure the length, surface area and volume of crown and root and to analyze the mathematical relation among crown-to-root ratios in terms of length, surface area and volume. Twenty-five premolars were scanned using micro-computed tomography (micro CT) in vitro to build 3D models. The long axis and enamelo-cemental junction of each tooth were determined with the help of Geomagic Studio software, and the length, surface and volume of crown and root were measured. The crown-to-root ratios in terms of length, surface and volume were calculated and the relationship among length, surface area and volume of crown and root as well their ratios were analyzed using SPSS software. The interrelationship among crown length (x), surface area (y) and volume (z) could be expressed as z= -808.2 0+ 124.80x +3.35y -5.59x2-0.14xy+3.47y 2*10-4 (R2 = 0.99) and that of root length (x1), surface area (y1) and volume (z1), as z1= -207.50 +13.87x1+1.75y1 + 5.03x12*10-2-8.05x 1y1 *10-2+ 2.58*10-3y12 (R2 = 0.93) . The correlation among crown-to-root ratio in length(x2), crown-to-root ratio in surface area (y2) and crown-to-root ratio in volume (z2) could be expressed in z2= -4.48*10-2 -1.25x2*10-2+1.20y2 + 3.29x22-5.05x2y2 + 2.00y22 (R2 = 0.96). The length, surface area and volume of crown and root of premolars share a close relationship, while, a definite mathematical relation could be observed amongst their ratios. Crown to root ratio in terms of length, surface and volume, may provide a novel multi-criterion method for evaluating tooth function.

El objetivo de este estudio fue construir imágenes tridimensionales (3D) de los dientes premolares para medir la longitud, superficie y volumen de la corona y raíz, junto con analizar la relación matemática entre las proporciones de la corona a la raíz en términos de longitud, superficie y volumen. Veinticinco premolares fueron escaneados mediante microtomografía computadorizada (microTC) in vitro para construir modelos en 3D. Con el software Geomagic se determinaron el eje y la unión amelo-cementaria de cada diente, y se midieron la longitud, superficie y volumen de la corona y la raíz de los dientes premolares. Con el programa SPSS se calcularon y analizaron las proporciones de la corona a la raíz en términos de longitud, superficie y volumen y la relación entre la longitud, superficie y volumen de la corona y de la raíz. La interrelación entre la longitud de la corona (x), superficie (y) y el volumen (z) puede ser expresado como z= -808,2 0+ 124,80x + 3,35y -5,59x2-0,14xy + 3.47y2*10-4 (R2= 0,99) y la de longitud de la raíz (x1), área de superficie (y1) y el volumen (z1), como z1= -207,50 + 13.87x1 + 1.75y1 + 5.03x12 * 10-2-8.05x1y1 * 10-2 + 2,58 * 10-3y12 (R2= 0,93). La correlación entre la relación de corona a raíz en longitud (x2), la relación corona a raíz en superficie (y2) y la relación corona a raíz en volumen (Z2) podría expresarse en z2 = -4,48 * 10-2 * 10-2 -1.25x2 + 1.20y2 3.29x22-5.05x2y2 + 2.00y22 (R2= 0,96). La longitud, superficie y volumen de la corona y la raíz de los dientes premolares comparten una estrecha relación, mientras que, una relación matemática definida se pudo observar entre sus proporciones. La relación entre la corona y raíz en términos de longitud, superficie y volumen, puede proporcionar un nuevo método multi-criterio para evaluar la función de los dientes.

Um método eficiente e ecologicamente amigável de hidrocianação de sulfoniliminas por um procedimento de duas etapas em uma única operação usando hexacianoferrato(II) de potássio como fonte de cianeto, cloreto de benzoíla como promotor e carbonato de potássio como uma base é descrito. Este método tem como característica o uso de fonte de cianeto atóxica, não volátil e barata, alto rendimento e um procedimento simples.

An efficient and eco-friendly method for hydrocyanation of sulfonylimines via one-pot two-step procedure using potassium hexacyanoferrate(II) as a cyanide source, benzoyl chloride as a promoter, and potassium carbonate as a base is described. This protocol has the features of using nontoxic, nonvolatile and inexpensive cyanide source, high yield, and simple work-up procedure.